Storage of buffalo (Bubalus bubalis) semen.
نویسندگان
چکیده
Characteristics of buffalo semen, diluents used for liquid storage, aspects involved in freezing and thawing of semen are reviewed, and fertility results after artificial insemination (AI) with frozen-thawed semen are given.
منابع مشابه
Cryopreservation of in situ cool stored buffalo (Bubalus bubalis) epididymal sperm
The objectives of this study were to investigate the effects of testis-epididymis cool storage on viability and progressive motility of buffalo epididymal sperm (EP) and to compare the influences of two basic semen extenders on post thaw EP viability and progressive motility. Abattoir collected buffalo testicles were allotted to three storage times (0 h: n = 10; 24 h: n = 10 and 48 h: n = 12). ...
متن کاملFactors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.
Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...
متن کاملGlutathione Addition in Tris-citric Egg Yolk Extender Improves the Quality of Cooled Buffalo (Bubalus bubalis) Bull Semen
The present study was designed to see whether adding glutathione to tris-citric egg yolk extender improves the quality of buffalo bull spermatozoa stored at 5°C for five days. Semen collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 5) was diluted (37C; 10 x 10 motile spermatozoa per ml) with extender having glutathione (0.0, 0.5, 1.0, 2.0 and 3.0mM). The extended semen was cooled fr...
متن کاملEFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL (Bubalus bubalis) SEMEN
The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...
متن کاملP-103: Effects of In Vitro Selenium Supplementation on Buffalo (Bubalus bubalis) Sperm Quality following Cryopreservation
Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...
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ورودعنوان ژورنال:
- Animal reproduction science
دوره 62 1-3 شماره
صفحات -
تاریخ انتشار 2000